Artificial Insemination
Information herein is provided to be used as guidelines for bovine.
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Keep breeding kit clean and organized.
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Identify cow(s) and check breeding record.
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Wash hands.
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Have sleeve, sheath, gun, scissors and towels ready
before thawing semen.
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Prepare water bath @ 95°F and place near tank.
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Keep semen below frost line while removing straws
for thawing.
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Remove straws within 10 seconds.
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Shake straw to remove excess Nitrogen and quickly
plunge into the water bath for 45 seconds.
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Insemination gun and sheath should also be
approximately 95°F and not extremely hot or cold to touch.
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Dry straw completely and check semen I.D.
prior to loading gun.
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Clip 1/4" squarely from the end of the straw and
apply sheath. Lock o-ring firmly in place.
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Wrap prepared gun in dry paper towel or protective
sheath and tuck close to the body.
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Clean vulva thoroughly prior to insertion of
breeding gun.
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Insert insemination gun at 30° angle to avoid
urethral opening.
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Pass rod through cervix, check location (uterine
body) with tip of index finger and slowly deposit 1/2 of semen.
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Recheck location and deposit rest of semen.
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Slowly remove gun and check for infection, blood and
semen feedback inside sheath.
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Recheck semen I.D.
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Properly dispose of sheath, towels and glove.
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Clean gun if needed.
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Record breeding information on barn chart and/or
other record keeping system.
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Recheck temperature of water bath prior to thawing
additional straws and repeat the steps listed above.
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Please click here to see the Heat Expectancy and
Gestation Chart as well as a chart of Gestation Variances.
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